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Hematoxylin 1
Q: Can Hematoxylin 1 be used in immunoperoxidase techniques?
A: Yes. Hematoxylin 1 does not contain alcohol.
Q: Does Hematoxylin 1 form a metallic sheen over time?
A: No. Hematoxylin 1 is stable and was developed to prevent the formation of
sheens.
Hematoxylin 2
Q: What is the benefit of Hematoxylin 2 versus Gill Hematoxylin or Harris
Hematoxylin?
A: Hematoxylin 2 will stain tissue as intense as Gill 3. Its advantages over Harris
Hematoxylin are that it does not form a metallic sheen, does not crystallize,
has a longer shelf life, and is much more consistent and stable.
Q: What are the differences between Hematoxylin 2 and Hematoxylin 1?
A: Hematoxylin 2 is formulated at almost twice the strength of Hematoxylin 1.
Therefore, laboratories requiring shorter stain times may like the rapid
staining results of Hematoxylin 2.
Hematoxylin 7211
Q: What is the benefit of Hematoxylin 7211 versus Gill Hematoxylin or Harris
Hematoxylin?
A: Hematoxylin 7211 is a hybrid of both Gill and Harris Hematoxylin It produces
the superb staining qualities of a Harris Hematoxylin along with the stable
and rapid qualities of a Gill Hematoxylin.
Q: Why are there crystals in the Hematoxylin 7211 bottle?
A: Hematoxylin 7211 uses aluminum ammonium sulfate at its saturation point to
maintain the highest amount of dye-lake complex for optimal staining. If the
crystals are troublesome, simply filter them out.
Q: Can Hematoxylin 7211 be used in immunoperoxidase techniques?
A: Yes. Hematoxylin 7211 does not contain alcohol.
Clarifier 1
Q: What is the difference between acid alcohol and Clarifier 1?
A: Differentiating with acid alcohol removes stain from the cytoplasm as well as
some from the nuclear chromatin. Clarifier 1 selectively removes background
staining caused by excess gelatin/adhesive used in the water bath.
Q: What is the role of Clarifier 1 in cytology staining?
A: Clarifier 1 acts as a selective, gentle reagent to render cells more
transparent. Additionally, it may be used to enhance green and red hues in
the cytoplasm.
Q: Can Clarifier 1 be used with Hematoxylin 2 in tissue staining?
A: No. Clarifier 1 is not strong enough to eliminate the background staining
produced by Hematoxylin 2.
Clarifier 2
Q: What is the difference between acid alcohol and Clarifier 2?
A: Differentiating with acid alcohol removes stain from the cytoplasm as well as
some from the nuclear chromatin. Clarifier 2 selectively removes background
staining caused by excess gelatin/adhesive used in the water bath.
Q: Can Clarifier 2 be used with Hematoxylin 1?
A: Yes.
Eosin-Y
Q: What adjustments can be made if the Eosin-Y stain is too bright?
A: Decrease the time in the Eosin-Y stain and/or add a 95% alcohol rinse
directly after the Eosin-Y stain to lessen the intensity.
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